Friday, February 9, 2018

Bioprotocols of Molecular Biology




One-Tube Isolation of DNA from Mouse Tails
Michael R. Green
   
and Joseph Sambrook
Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot093534
   
   
Buffers
Michael R. Green
   
and Joseph Sambrook
Cold Spring Harb Protoc 2018; doi:10.1101/pdb.top098210
   
   
Caring for Escherichia coli
Michael R. Green
   
and Joseph Sambrook
Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot101337
   
   
Protein Immunodepletion and Complementation in Xenopus laevis Egg Extracts
Christopher Jenness, 

David J. Wynne, 

and Hironori Funabiki
Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot097113
   
Analysis of Mitotic Checkpoint Function in Xenopus Egg Extracts
Yinghui Mao
Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot099853
   
Transformation of Schizosaccharomyces pombe in a 96-Well Format
Assen Roguev, 

Jiewei Xu, 

and Nevan J. Krogan
Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot091942
   
   
DNA Preparation from Schizosaccharomyces pombe
Assen Roguev, 

Jiewei Xu, 

and Nevan J. Krogan
Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot091959
   
   
Preparation of Plasmid DNA by Alkaline Lysis with Sodium Dodecyl Sulfate: Maxipreps
Michael R. Green
   
and Joseph Sambrook
Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot093351
   
   
A Single-Step Method for the Simultaneous Preparation of DNA, RNA, and Protein from Cells and Tissues
Michael R. Green
   
and Joseph Sambrook
Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot093500
   
   
Gateway Recombinational Cloning
John S. Reece-Hoyes
   
and Albertha J.M. Walhout
Cold Spring Harb Protoc 2018; doi:10.1101/pdb.top094912
   
   


Propagating Gateway Vectors

John S. Reece-Hoyes
   
and Albertha J.M. Walhout
Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot094920
   
   
Generating an Open Reading Frame (ORF) Entry Clone and Destination Clone
John S. Reece-Hoyes
   
and Albertha J.M. Walhout
Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot094938
   
   
Using Multisite LR Cloning to Generate a Destination Clone
John S. Reece-Hoyes
   
and Albertha J.M. Walhout
Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot094946
   
   
Preparation of DNA from Embryonic Stem Cells or Other Cultured Cells
Richard Behringer, 

Marina Gertsenstein, 

Kristina Vintersten Nagy, 

and Andras Nagy
Cold Spring Harb Protoc 2017; doi:10.1101/pdb.prot092718
   
   
Analysis of DNA Methylation in Mammalian Cells
Paul M. Lizardi, 

Qin Yan, 

and Narendra Wajapeyee
Cold Spring Harb Protoc 2017; doi:10.1101/pdb.top094821
   
   
Methylation-Specific Polymerase Chain Reaction (PCR) for Gene-Specific DNA Methylation Detection
Paul M. Lizardi, 

Qin Yan, 

and Narendra Wajapeyee
Cold Spring Harb Protoc 2017; doi:10.1101/pdb.prot094847
   
   
Methyl-Cytosine-Based Immunoprecipitation for DNA Methylation Analysis
Paul M. Lizardi, 

Qin Yan, 

and Narendra Wajapeyee
Cold Spring Harb Protoc 2017; doi:10.1101/pdb.prot094854
   
   
High-Throughput Deep Sequencing for Mapping Mammalian DNA Methylation
Paul M. Lizardi, 

Qin Yan, 

and Narendra Wajapeyee
Cold Spring Harb Protoc 2017; doi:10.1101/pdb.prot094862
   
   
Preparation of Single-Stranded Bacteriophage M13 DNA by Precipitation with Polyethylene Glycol
Michael R. Green
   
and Joseph Sambrook
Cold Spring Harb Protoc 2017; doi:10.1101/pdb.prot093419
   
   
Growing Bacteriophage M13 in Liquid Culture
Michael R. Green
   
and Joseph Sambrook
Cold Spring Harb Protoc 2017; doi:10.1101/pdb.prot093435
   
   


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