Chapter 1. Basic Lab Technology

Alternate method for purifying DNA from agarose gels

Isolation of RNA from mammalian cells using RNAZOL (Teltest)

Preparation of sequencing gels

Preparing proteins/antibodies/DNA/RNA for microinjection

RT-PCR Protocol

Safety Procedures
Preparation of Solutions
Disposal of Buffers and Chemicals
Equipment

Southern blotting

Transfection of mammalian cells using Lipofectamine (LTI)

Transformation of E. coli by electroporation

Wizard PCR preps DNA purification system

Working with DNA
Sterile Technique