Chapter.10. Cellular Biology 1

Aseptic Cell Transfers

Calcium Phosphate tranfection of adherent mammalian cells (works well for 293 cells)

Cell mass by Measurement of Turbidity

Computation of Transfer Aliquots

Culturing MEFs
Calcium Phosphate Coprecipitation

Determining cell counts

Effectene (QIAGEN) tranfection of adherent mammalian cells (works well for HeLas and many other cell types)

Establishing stable cell lines

Establishment of a Primary Culture

Eukaryote Growth Dynamics

Examination of Bacterial Colonies

Fugene (Roche) tranfection of adherent mammalian cellsDOTAP (Roche) tranfection of adherent mammalian cells

Fusing cells to create heterokaryons