Chapter 5. Cell Fractionation

Cell Fractionation Protocol.
Chromatin isolation
Spliceosome purification

Cell Fractionation Protocol.
Covalently conjugate antibodies to beads for affinity purification
Nucleolar isolation (contains images and movies)
Cajal body isolation (contains images and movies)
Lysing cells for release of both cytoplasmic and nuclear proteins

Equilibrium Density Gradient - Percoll

Low Speed Separaton of Cells

Preparation of nuclear extract from plated cells

Sedimentation Densities

Separation of Cells by Velocity Sedimentation

Sucrose Fractionation of Castor Bean