Saturday, December 20, 2008

Chapter.15. BACTERIA

Electro Competent Agrobacterium Cells

Electroporation

Introduction of plasmid into Agrobacterium (method II)

Introduction of plasmid into Agrobacterium (method III)
General Cloning Procedures
Colony Hybridization
Electroporation & Blue/White Selection
Large scale plasmid prep, PEG method
Large scale plasmid prep, Qiagen/CsCl
Ligation Optimization
Plasmid Miniprep

Preparation of competent E. coli cells using CaCl2

Transformation of bacteria
Release of recombinant proteins from bacterial cells using Novagen's Bugbuster

Purification of recombinant hexahistidine-tagged proteins

Purification of recombinant GST-tagged proteins

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