Affinity column preparation and Immunoprecipitation
Affinity purification of Antibodies
B-PER Bacterial Protein extraction reagent (Pierce)
Bradford quantification of protein concentration
Crosslinking the antibody to the beads protocol | Abcam
Factor Xa Cleavage of GST fusions
GST-fusion protein injection into rabbits
GST-protein fusion expression, purification and other uses
His-fusion protein purification (Novagen/Qiagen)
Histone Kinase Assay
Western blot
p27 ELISA
Protein Interactions Captured by Chemical Cross-linking: One-Step Cross Linking.
Protein preparation (from plants tissue)
Protocol: Cross-linking of IgG to Protein A or G Beads: NEB
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